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KMID : 0360320000320020304
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Journal of Korean Cancer Research Association
2000 Volume.32 No. 2 p.304 ~ p.311
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Detection of Circulating Tumor Cells in Peripheral Blood by Reverse Transcriptase - polymerase Chain Reaction in Gastric Cancer Patients
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Kim Hawk
Lee Young-Yiul
Jeong Tae-June
Choi Il-Young
Kim In-Soon
Han Dong-Soo
Jeon Yong-Chul
Sohn Joo-Hyun
Park Hwon-Kyum
Baik Hong-Kyu
Lee Hong-Gee
Nam Young-Soo
Ahn Myung-Ju
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Abstract
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Purpose :Cancer cells can be detected in bloods, lymph nodes or bone marrows by using reverse transcriptase-polymerase chain reaction (RT-PCR). We investigated to detect carcinoembryonic antigen (CEA) mRNA in peripheral blood by RT-PCR as a circulating tumor cell marker of gastric
cancer patients.
Materials and Methods :Gastric cancer patients were early gastric cancer with curative surgery (Group A, n=9), advanced gastric cancer with curative surgery (Group B, n=18) and relapsed or metastatic gastric cancer (Group C, n=13). RT-PCR was performed to detect CEA mRNA expression in the peripheral blood mononuclear cells and we used Colo 201 cells as a positive control.
Results :Seventeen patients (42.5%) were positive for CEA mRNA, whereas, all the nine normal subjects were negative. There were significant differences between group A and C (p=0.041), group B and C (p=0.001) and between patients underwent curative surgery and metastatic gastric cancer patients (p=0.001) but not between A and B (p=0.036) for the positive rate of CEA mRNA.
Conclusion :Large number of gastric cancer patients showed positive CEA mRNA in peripheral blood suggesting that gastric cancer cells can metastasize into blood at early stage.
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KEYWORD
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Stomach neoplasm, Micrometastasis, CEA,
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